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Test instructions: Dengue Fever (Anti-Dengue IgM/IgG)

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CONTENT OF THE KIT

  1. Test cassette
  2. Pipette
  3. Bottle of buffer solution
  4. Sterile safety lancet
  5. Alcohol pad

PRECAUTIONS

  1. Carefully follow the instructions exactly.
  2. Only use supplied materials.
  3. Wash the hands thoroughly to avoid contamination of the specimen. Contamination may cause erroneous result.
  4. This kit is for in vitro use only. Do not swallow.
  5. Discard after first use. The test can only be used once.
  6. Do not use test kit beyond the expiration date.
  7. Do not use the kit if the pouch is punctured or not well sealed.
  8. Keep out of children's reach.

STORAGE AND STABILITY

  1. Store at 4°C to 30°C in the sealed pouch up to expiration date.
  2. Keep away from sunlight, moisture and heat.
  3. Use the test shortly after opening the pouch.

TEST PROCEDURE

  1. When the test have been stored in a cold room, wait for it to reach room temperature (10 - 30°C).
  2. Open the pouch and place the cassette on a level surface.
  3. Clean the area to be lanced with the alcohol pad. Wait for the area to dry completely (do not blow on it).
  4. Use the safety lancet and pipette. The safety lancet can only be used once.
    Twist off the green protective cap, and then pull it straight out
    Press the lancet firmly against the puncture site to activate the device
    Gently apply intermittent pressure near the puncture site to obtain the required blood volume

    Squeeze the pipette together and collect blood by slowly releasing the pressure.

  5. Add One drop of sample, from the pipette, to the round well of the cassette (marked with S).
  6. Add Two drops of buffer solution, from the bottle, to the round well of the cassette (marked with D).

HOW TO INTERPRET THE RESULT

Read the test result after 15 minutes. Do not read the result after 20 minutes.

Positive (Dengue Fever infection):

Control line and at least one test line appear on the membrane. The appearing of T1 test line indicates a DV IgM positive result, the appearing of T2 test line indicates a DV IgG positive result. The appearance of both T1 and T2 test lines indicate positive result for both DV IgM and DV IgG.

Negative (No Dengue Fever infection):

Only the black control band appears on the membrane. The absence of a test band indicates a negative result.

Invalid:

There should always be a black control band in the control region regardless of test result. If control band is not seen, the test is considered invalid. It is normal to have a slightly lightened control band with very strong positive samples as long as it is distinctly visible.

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